About

Introduction

This website contains a collection of publicly available datasets used by the Hemberg Group at the Sanger Institute.

SingleCellExperiment and scater

We use SingleCellExperiment Bioconductor S4 class to store our data and scater for quality control and plotting purposes. For each dataset you can find both a SingleCellExperiment object and a scater report.

Contributions

We welcome contributions to our collection. Please create a pull request to our GitHub repository providing the following information:

  • bash script with downloading and processing instructions named as the dataset first author's surname.
  • R script file with the instruction on how to create a SingleCellExperiment object named as the dataset first author's surname.

Note

The cell type information must be located in the cell_type1 column of the colData slot of the SingleCellExperiment object. If there are more than one cell type hierarchies please use cell_type2, cell_type3 etc.

  • Metadata information about the dataset: publication reference, accession, protocol and size of the dataset. These should be added in markdown format to the existing files in the website folder or by creating a new markdown file.

scmap

Some of the dataset hosted here are used as references for scmap, our web-based application for fast unsupervised projection of single cell RNA-seq data.

Contacts

Please send you feedback/comments/suggestions to Vladimir Kiselev.